p ip3r  (Proteintech)

Journal: Bioactive Materials

Article Title: Amorphous calcium zinc phosphate promotes macrophage-driven alveolar bone regeneration via modulation of energy metabolism and mitochondrial homeostasis

doi: 10.1016/j.bioactmat.2025.06.053

Figure Lengend Snippet: ACZP reverses the pro-inflammatory polarization phenotype of macrophages induced by HA through inhibiting the IP3R/MCU axis: (A) GO bubble plots showed the calcium ion transport-related biological function differences of macrophages between the HA and Control groups. Dot size and color correspond to the enrichment P -value of that term (after -log10 transformation). (B) Heatmap of the expression of calcium ion transport-related genes in all macrophage clusters between the HA and Control groups. (C) Violin plot of the expression of the Micu1 gene in macrophages of the HA and Control groups. (D) UMAP atlas showed the expression of Micu1 in macrophages of the HA and Control groups. (E) Two-dimensional image of IP3R and MCU molecular with or without HA or ACZP docking simulation. (F) Representative images of the co-localization of the mitochondria and ER in macrophages stained with the Mito-Tracker and ER-Tracker (green: mitochondria; red: ER; scale bar: 10 μm; 2 μm (inset); The arrow pointed to the MAMs structure.). (G) Immuno-electron microscopy showed the expression of IP3R and MCU antibodies between the mitochondria and ER. (scale bar: 500 nm). (H) The morphology of the mitochondria and ER of Control, HA and ACZP groups were analyzed by TEM, and the representative images were displayed. (green: mitochondria; purple: ER; scale bar: 2 μm (left); 500 nm (right)). (I–J) The distance between the ER and mitochondria (n = 30) and the mitochondrial perimeter (n = 30) were quantified and analyzed, respectively. The data were shown as the mean ± SD; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significant differences between the indicated columns (one-way ANOVA). (K) Schematic diagram of the calcium ion transport channel between the mitochondria and ER in macrophages.

Article Snippet: Sections were incubated overnight with specific antibodies (CD68, MCU, IP3R; Scanco Medical AG, ZH, CH) at 4 °C.

Techniques: Control, Transformation Assay, Expressing, Staining, Immuno-Electron Microscopy

Journal: Bioactive Materials

Article Title: Amorphous calcium zinc phosphate promotes macrophage-driven alveolar bone regeneration via modulation of energy metabolism and mitochondrial homeostasis

doi: 10.1016/j.bioactmat.2025.06.053

Figure Lengend Snippet: ACZP promotes the repair of alveolar bone defects in New Zealand white rabbits: (A) Schematic diagram of nanomaterials for treating rabbit alveolar bone defects. (B) Representative Micro-CT, H&E, and Masson staining of rabbit alveolar bone 4 weeks after implantation in the Control group, HA group and ACZP group (scale bar: 1 mm (Micro-CT); 500 μm (H&E and Masson staining)). (C) Representative Micro-CT, H&E, and Masson staining of rabbit alveolar bone 12 weeks after implantation in the Control group, HA group and ACZP group (scale bar: 1 mm (Micro-CT); 500 μm (H&E and Masson staining)). (D–E) Semi-quantification of BV/TV, Tb.N, Tb.Th and Tb.Sp in different groups at 4 (left) and 12 (right) weeks. ( n = 3). The data were shown as the mean ± SD ( n = 3); ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significant differences between the indicated columns (one-way ANOVA). (F) Representative immunofluorescence image of MCU staining (yellow) and IP3R staining (green) in alveolar bone defect after 4 weeks of implantation. (scale bar: 200 μm).

Article Snippet: Sections were incubated overnight with specific antibodies (CD68, MCU, IP3R; Scanco Medical AG, ZH, CH) at 4 °C.

Techniques: Micro-CT, Staining, Control, Immunofluorescence